| Houston Hurricanes FC | — | December 2012 | Lutheran South Academy |
| Idaho Wolves | — | 2004 | — |
| Indianapolis Braves | — | 2007 | — |
| Inter Nashville FC | Nashville | — | — |
| Kalamazoo FC | Kalamazoo | 2015 | — |
| Kingston Stockade Football Club | Kingston | 2015 | Dietz Stadium |
| Knoxville Force | Knoxville | 2010 | — |
| Kraze United | — | 2015 | UCF Soccer and Track Complex |
| Lancaster Inferno | — | 2008 | Hempfield High School |
| Las Vegas Strikers | — | 2003 | — |
| Long Island Academy | — | 2006 | — |
| Madison 56ers | Madison | 2005 | Breese Stevens Field |
| Maine Sting | — | 2008 | — |
| Mass United FC | — | 2009 | — |
| Miami Fusion FC | — | 2014 | — |
| Milwaukee Torrent | Milwaukee | January 2015 | — |
| Minneapolis City SC | Minneapolis | 2015 | — |
| Minnesota Kings | — | 2009 | — |
| Minnesota Twin Stars | — | 1997 | Minnetonka High School |
| Mississippi Storm | — | 2011 | — |
| Morris County Colonials | — | 2007 | — |
| Myrtle Beach FC | Myrtle Beach | 2011 | Doug Shaw Memorial Stadium |
| Nashville FC | — | 2013 | FirstBank Stadium |
| New Hampshire Mountaineers | — | 2009 | — |
| New Jersey Blaze | — | 2009 | Rowland Park |
| New Orleans Jesters | New Orleans | 2003 | Pan American Stadium |
| New York Cosmos B | — | 2015 | Mitchel Athletic Complex |
| NorCal Lamorinda United SC | — | 2009 | Miramonte High School |
| Northern Nevada Aces | — | 2001 | — |
| NSC United | — | 2005 | — |
| Oklahoma City FC | — | 2012 | Oklahoma City University |
| Panathinaikos Chicago | Naperville | 2020 | — |
| PDX FC | Portland | 2017 | — |
| Pennsylvania Stoners | Allentown | 2007 | Whitehall High School |
| Philadelphia Lone Star FC | Philadelphia | 2001 | — |
| Phoenix Banat Storm | — | 2006 | — |
| Phoenix Monsoon | Arizona | 2010 | — |
| Pocono Snow | — | 2008 | — |
| Port City FC | — | 2016 | — |
| Pumas FC | Birmingham | 1999 | — |
| Real San Jose | San Jose | 2007 | PAL Stadium |
| Real Shore F.C. | — | 2006 | — |
| Redwood City Ruckus | — | 2006 | — |
| RI Reds | Rhode Island | 2012 | — |
| Rio Grande Valley Ocelots FC | — | 2010 | — |
| Rochester Med City FC | Rochester | — | — |
| Rocket City United | — | 2007 | — |
| RVA FC | Fredericksburg | 2013 | Sports Backers Stadium |