| AC Crusaders | — | 2010 | Egg Harbor Township High School |
| AFC Ann Arbor | Ann Arbor | 2014 | — |
| AFC Cleveland | Independence | November 11, 2011 | Independence High School |
| Albion SC San Diego | San Diego | 2015 | Mission Bay High School |
| Albuquerque Asylum | — | 2004 | Menaul School |
| Asheville City SC | Asheville | 2016 | Memorial Stadium, Asheville |
| Atlanta Silverbacks Reserves | Atlanta | 2007 | Atlanta Silverbacks Park |
| Atlantic City Diablos | — | 2007 | St. Augustine College Preparatory School |
| Bavarian United SC | Milwaukee | 1929 | — |
| Bay Area Ambassadors | — | 2009 | Chabot College |
| Boca Raton FC | Boca Raton | 2015 | Atlantic Community High School |
| Boston Aztec | — | 2006 | — |
| Brooklyn Italians | New York City | 1949 | John Dewey High School |
| Buffalo City FC | — | 2008 | Nichols School |
| Buxmont Torch FC | Perkasie | 2010 | Christopher Dock Mennonite High School |
| Cape Coral Hurricanes | — | 2010 | Cape Coral High School |
| CD Aguiluchos USA | Oakland | 2012 | — |
| Charlottesville Alliance FC | Charlottesville | 2016 | Charlottesville High School |
| Charm City FC | — | 2007 | Arundel High School |
| Chesterfield United FC | — | 2013 | Williams Stadium |
| Chicago Fire NPSL | — | 2007 | SeatGeek Stadium |
| Chico Rooks | Chico | 1993 | University Soccer Stadium, Chico |
| Cleveland SC | Cleveland | 2018 | Don Shula Stadium |
| Colorado Crimson | — | 2007 | — |
| Dakota Fusion FC | Fargo | — | — |
| Denver Kickers | — | 1962 | — |
| Detroit Arsenal | Berkley | 1997 | — |
| Duluth FC | Duluth | 2015 | Denfeld High School |
| Eau Claire Aris FC | — | 2009 | — |
| El Farolito Soccer Club | San Francisco | 1985 | Boxer Stadium |
| Erie Admirals S.C. | Erie | 2009 | — |
| F.C. New York | Queens | 2009 | Belson Stadium |
| FC Arizona | Mesa | 2016 | — |
| FC Columbus | Columbus | — | — |
| FC Force | — | 2014 | Central Union High School |
| FC Monmouth | Monmouth County | 2017 | — |
| FC Motown | Madison | 2012 | — |
| FC New Orleans | — | 2012 | — |
| FC Santa Clarita | — | 2006 | — |
| FC Sonic Lehigh Valley | Whitehall | 2009 | J. Birney Crum Stadium |
| FC Tacoma 253 | — | 2014 | — |
| FC Wichita | Wichita | 2013 | — |
| Galveston Pirate SC | — | 2010 | — |
| Georgia Revolution | McDonough | 2010 | Henry County High School |
| Grand Rapids Alliance | — | 2002 | — |
| Hartford City FC | Hartford | 2015 | — |
| Hershey Soccer Club | Hershey | 2013 | — |
| Hollywood United Hitmen | — | 2009 | Crenshaw High School |