| AC Connecticut | Newtown | 2011 | — |
| Ajax Orlando Prospects | Orlando | 2002 | Warden Stadium |
| Akron Summit Assault | — | 2010 | St. Vincent–St. Mary High School |
| Albany BWP Highlanders | — | 2003 | — |
| Atlanta Blackhawks | — | 2009 | Alpharetta High School |
| Atlanta Silverbacks U23's | — | 2006 | Atlanta Silverbacks Park |
| Augusta FireBall | — | 2005 | Patriots Park |
| Austin Aztex | Austin | 2011 | House Park |
| Austin Aztex U23 | — | 2007 | Round Rock High School |
| Bakersfield Brigade | — | 2005 | Bakersfield Christian High School |
| Ballard FC | Seattle | — | — |
| Baltimore Bohemians | — | 2011 | Cedar Lane |
| Bermuda Hogges | Pembroke Parish | 2006 | Bermuda National Stadium |
| Birmingham Hammers | Birmingham | 2013 | — |
| Boston Bolts | — | 2015 | — |
| Boston Victory S.C. | — | 2011 | Veterans Memorial Stadium |
| Brave SC | The Villages | 2008 | — |
| Brazos Valley Cavalry F.C. | Bryan | 2017 | Travis Field |
| Brooklyn Knights | — | 1999 | Metropolitan Oval |
| Burlingame Dragons FC | Burlingame | 2014 | Burlingame High School |
| Caledonia SC | Lakeland | January 12, 2022 | — |
| California Gold | — | 2002 | — |
| Cape Cod Crusaders | Massachusetts | 1994 | Massachusetts Maritime Academy |
| Cascade Surge | — | 1995 | McCulloch Stadium |
| Central Coast Roadrunners | San Luis Obispo | 1996 | Mustang Memorial Field |
| Charlotte Eagles | Charlotte | 1991 | Sportsplex at Matthews |
| Charlotte Independence Soccer Club | Rock Hill | — | Manchester Meadows Soccer Complex |
| Chesapeake Dragons | — | 2001 | Maryland SoccerPlex |
| Chicago Fire U-23 | — | 2001 | SeatGeek Stadium |
| Chicago Inferno | — | 2011 | — |
| Cincinnati Dutch Lions | — | 2013 | — |
| Cincinnati Kings | Cincinnati | 2005 | — |
| Cincinnati Riverhawks | — | 1997 | — |
| Cleveland Internationals | — | 2004 | — |
| Cocoa Expos | Cocoa | 1993 | — |
| Colorado Springs Ascent | — | 2000 | Switchbacks Training Stadium |
| Columbus Shooting Stars | — | — | Historic Crew Stadium |
| D.C. United U-23 | — | 2011 | Maryland SoccerPlex |
| Dayton Gemini | — | 2000 | Baujan Field |
| Delaware Dynasty | — | 2006 | — |
| Denver Cougars | — | 1998 | — |
| Des Moines Menace | Des Moines | 1994 | Valley High School |
| DFW Tornados | Bedford | 1986 | Toyota Stadium |
| El Paso Patriots | El Paso | 1989 | Patriot Stadium |
| Evergreen FC | Leesburg | 2015 | — |
| FC Buffalo | Buffalo | 2009 | Buffalo State University |
| FC JAX Destroyers | — | 2010 | D. B. Milne Field |
| FC Miami City | Miami | 2014 | Tropical Park Stadium |