| Atlético Dallas | Dallas | 2024 | — |
| Austin Aztex | Austin | 2011 | House Park |
| Austin Bold FC | Austin | August 9, 2017 | Bold Stadium |
| Birmingham Legion FC | Birmingham | 2017 | PNC Field |
| Buffalo Pro Soccer | Buffalo | 2024 | Queen City Field |
| California Jaguars | — | 1995 | Salinas Sports Complex |
| Charleston Battery | Charleston | 1993 | MUSC Health Stadium |
| Charlotte Independence | Charlotte | 2014 | Sportsplex at Matthews |
| Colorado Springs Switchbacks FC | Colorado Springs | 2013 | Switchbacks Training Stadium |
| Columbia Heat | — | 1993 | Eugene E. Stone III Stadium |
| Detroit City FC | Detroit | 2012 | Keyworth Stadium |
| El Paso Locomotive FC | El Paso | March 1, 2018 | New El Paso Ballpark |
| F.C. New York | Queens | 2009 | Belson Stadium |
| FC Tulsa | Tulsa | December 2013 | Oneok Field |
| Fresno FC | Fresno | July 26, 2017 | Chukchansi Park |
| Hartford Athletic | Hartford | July 11, 2018 | Trinity Health Stadium |
| Indy Eleven | Indianapolis | 2013 | IU Michael A. Carroll Track & Soccer Stadium |
| Las Vegas Lights FC | Las Vegas | August 11, 2017 | Cashman Field |
| Loudoun United FC | Leesburg | July 18, 2018 | Audi Field |
| Louisville City FC | Louisville | June 4, 2014 | Lynn Family Stadium |
| Memphis 901 FC | Memphis | January 8, 2018 | AutoZone Park |
| Miami FC | Miami | 2015 | Pitbull Stadium |
| Nashville SC | Nashville | May 19, 2016 | First Horizon Park |
| New Mexico United | Albuquerque | June 6, 2018 | Isotopes Park |
| North Carolina FC | Cary | January 26, 2006 | WakeMed Soccer Park |
| Oakland Roots SC | Oakland | 2018 | Oakland Coliseum |
| OKC Energy FC | Oklahoma City | July 2, 2013 | Taft Stadium |
| Orlando City SC | — | 2010 | Camping World Stadium, ESPN Wide World of Sports Complex |
| Phoenix FC | Phoenix | July 2, 2012 | Sun Devil Soccer/Lacrosse Stadium |
| Phoenix Rising FC | Peoria | March 13, 2014 | Phoenix Rising Soccer Stadium, Peoria Sports Complex, Casino Arizona Field, Scottsdale Stadium |
| Pittsburgh Riverhounds SC | Pittsburgh | March 11, 1998 | Highmark Stadium |
| Puerto Rico United | Aguada | 2007 | Aguada Stadium |
| Queensboro FC | Queens | November 12, 2019 | — |
| Reno 1868 FC | Reno | September 16, 2015 | Greater Nevada Field |
| Rio Grande Valley FC Toros | Edinburg | July 15, 2015 | Robert and Janet Vackar Stadium |
| Sacramento Republic FC | Sacramento | December 3, 2012 | Heart Health Park |
| San Diego Loyal SC | San Diego | 2019 | Torero Stadium |
| Sporting Club Jacksonville men | — | August 30, 2022 | — |
| Tampa Bay Rowdies | St. Petersburg | June 18, 2008 | George M. Steinbrenner Field, Al Lang Stadium |
| USL Chicago | Chicago | November 21, 2017 | Lincoln Yards Stadium |
| VSI Tampa Bay FC | Plant City | November 16, 2011 | Plant City Stadium |
| Wilmington Hammerheads FC | Wilmington | 1996 | Legion Stadium |