| Academia Quintana | — | 1969 | Hiram Bithorn Stadium |
| Atlético de San Juan FC | San Juan | 2008 | Hiram Bithorn Stadium |
| Atléticos de Levittown FC | — | 1989 | — |
| Ballista FC | — | 2016 | — |
| Caguas Sporting FC | — | 2016 | — |
| Club de Fútbol Fraigcomar | — | 1986 | — |
| Fajardo FC | — | 2010 | — |
| FC Leones | — | 2011 | Estadio Francisco Montaner |
| Gigantes de Carolina FC | Carolina | 2008 | Roberto Clemente Stadium |
| Guayama FC | — | 1949 | — |
| Guaynabo Fluminense FC | Guaynabo | 2002 | Estadio José "Pepito" Bonano |
| Huracán FC de Caguas | Caguas | 1991 | Parque Yldefonso Solá Morales |
| Mayagüez FC | — | 2004 | Mayagüez Athletics Stadium |
| Pachuca Puerto Rico | — | 2009 | Nestor Morales Stadium |
| Puerto Rico United | Aguada | 2007 | Aguada Stadium |
| Real Madrid | — | 2009 | Juan Ramón Loubriel Stadium |
| Tornados de Humacao | Humacao | 1994 | Nestor Morales Stadium |
| Yabuco SUAL FC | — | 2014 | — |