| Club | Based in | Founded | Home ground |
|---|---|---|---|
| Charlotte Independence | Charlotte | 2014 | Sportsplex at Matthews |
| Chattanooga Red Wolves SC | Chattanooga | August 1, 2018 | Chattanooga Christian School |
| FC Naples | — | January 2024 | — |
| FC Tucson | Arizona | 2010 | Veterans Memorial Stadium |
| Forward Madison FC | Madison | May 17, 2018 | Breese Stevens Field |
| Greenville Triumph SC | Greenville | March 13, 2018 | — |
| Lansing Ignite FC | Lansing | October 25, 2018 | Jackson Field |
| New York Cosmos | Paterson | 2025 | Hinchliffe Stadium |
| Northern Colorado Hailstorm FC | — | January 12, 2021 | — |
| One Knoxville SC | Knoxville | 2021 | — |
| Portland Hearts of Pine | — | September 7, 2023 | Fitzpatrick Stadium |
| Richmond Kickers | Richmond | 1993 | City Stadium |
| Sarasota Paradise | Lakewood Ranch | November 19, 2022 | — |
| Spokane Velocity | Spokane | 2021 | — |
| Tormenta FC | Statesboro | 2015 | Bo Pitts Field |
| Union Omaha | Omaha | 2019 | — |