| Club | Based in | Founded | Home ground |
|---|---|---|---|
| Brooklyn FC | Brooklyn | — | — |
| Buffalo Pro Soccer | Buffalo | 2024 | Queen City Field |
| Carolina Ascent FC | Charlotte | — | — |
| Fort Lauderdale United FC | Fort Lauderdale | November 21, 2023 | — |
| Sporting Club Jacksonville women | — | May 16, 2023 | — |