| Atlanta Beat | Women's United Soccer Association | Atlanta | Herndon Stadium |
| Carolina Courage | Women's United Soccer Association | Cary | WakeMed Soccer Park |
| Chesapeake Dragons | USL League Two | — | Maryland SoccerPlex |
| Chicago Fire U-23 | USL League Two | — | SeatGeek Stadium |
| Greenville Lions | USL League Two | — | Sirrine Stadium |
| Jersey Falcons | USL League Two | — | — |
| KC Athletics | United States Adult Soccer Association | — | — |
| MagicJack | Women's Professional Soccer | Boca Raton | — |
| Northern Nevada Aces | National Premier Soccer League | — | — |
| Philadelphia Lone Star FC | National Premier Soccer League | Philadelphia | — |
| Portland Timbers | A-League | — | Providence Park |
| Puget Sound Soccer Academy Rapids | Pacific Coast Soccer League | — | — |
| Quality Distributors | Guam Men's Soccer League | Guam | — |
| Sacramento Pride | Women's Premier Soccer League | — | — |
| San Francisco City FC | USL League Two | San Francisco | Negoesco Stadium |
| San Jose CyberRays | Women's United Soccer Association | San Jose | CEFCU Stadium |
| Sioux Falls Spitfire | USL League Two | — | O'Gorman Catholic High School |
| Southern California Seahorses | USL League Two | La Mirada | — |
| Washington Freedom | Women's Professional Soccer | — | Maryland SoccerPlex |
| West Dallas Kings | USL League Two | — | — |