| Arizona Impact | Premier Arena Soccer League | — | — |
| Birmingham Hammers | USL League Two | Birmingham | — |
| Chesterfield United FC | National Premier Soccer League | — | Williams Stadium |
| Cincinnati Dutch Lions | USL League Two | — | — |
| Cleveland Freeze | Major Arena Soccer League | — | — |
| Colorado Springs Switchbacks FC | USL Championship | Colorado Springs | Switchbacks Training Stadium |
| FC Tulsa | USL Championship | Tulsa | Oneok Field |
| FC Wichita | National Premier Soccer League | Wichita | — |
| Greater Lowell United FC | — | — | Cawley Memorial Stadium |
| Hershey Soccer Club | National Premier Soccer League | Hershey | — |
| Houston Dash | National Women's Soccer League | Houston | Shell Energy Stadium |
| Indy Eleven | USL Championship | Indianapolis | IU Michael A. Carroll Track & Soccer Stadium |
| Jacksonville Armada FC | North American Soccer League | — | VyStar Ballpark |
| Laredo Honey Badgers | Major Arena Soccer League | — | Laredo Energy Arena |
| Nashville FC | National Premier Soccer League | — | FirstBank Stadium |
| New York City FC | Major League Soccer | New York City | Yankee Stadium |
| OKC Energy FC | USL Championship | Oklahoma City | Taft Stadium |
| Orlando City SC | Major League Soccer | Orlando | Inter&Co Stadium |
| Peachtree City MOBA | USL League Two | Peachtree City | — |
| Pennsylvania Roar | Major Indoor Soccer League | — | Sovereign Center |
| RVA FC | National Premier Soccer League | Fredericksburg | Sports Backers Stadium |
| San Jose Earthquakes U23 | USL League Two | — | — |
| St. Louis Ambush | Major Arena Soccer League | — | Family Arena |
| Tobacco Road FC | USL League Two | — | Durham County Stadium |