| Arizona Thunder | World Indoor Soccer League | Arizona | Arizona Veterans Memorial Coliseum |
| Denver Cougars | USL League Two | — | — |
| IMG Academy Bradenton | USL League Two | Bradenton | — |
| Indiana Invaders | USL League Two | — | Indiana Invaders Soccer Complex |
| Mississippi Beach Kings | Eastern Indoor Soccer League | — | Mississippi Coast Coliseum |
| Northern Arizona Prospectors | USL League Two | Arizona | Bradshaw Mountain High School |
| Northern Virginia FC | USL League Two | — | — |
| Orlando City U-23 | USL League Two | Lake Mary | Seminole Soccer Complex |
| Pensacola Flyers | Eastern Indoor Soccer League | — | Pensacola Civic Center |
| Pittsburgh Riverhounds SC | USL Championship | Pittsburgh | Highmark Stadium |
| Portland Pythons | World Indoor Soccer League | — | Moda Center |
| Riverside County Elite | USL Second Division | — | West Valley High School |
| San Diego Flash | A-League, National Premier Soccer League | San Diego | Mira Mesa Senior High School |
| St. Louis Steamers | World Indoor Soccer League | — | Enterprise Center |
| Staten Island Vipers | A-League | Staten Island | Tottenville High School |
| Tallahassee Tempest | — | Tallahassee | — |
| Western Mass Pioneers | USL League Two | Ludlow | Lusitano Stadium |
| Wisconsin Rebels | USL League Two | — | — |