| Baton Rouge Bombers | Eastern Indoor Soccer League | — | Baton Rouge River Center Arena |
| Chicago Fire FC | Major League Soccer | Chicago | Soldier Field |
| Christos FC | — | Baltimore | — |
| Cincinnati Riverhawks | USL League Two | — | — |
| Detroit Arsenal | National Premier Soccer League | Berkley | — |
| Doxa Italia | United States Adult Soccer Association | — | — |
| Guam Shipyard | Guam Men's Soccer League | Tamuning | Guam National Football Stadium |
| Hershey Wildcats | A-League | — | Hersheypark Stadium |
| Huntsville Fire | Eastern Indoor Soccer League | — | Von Braun Center |
| Indiana Blaze | USL W-League | — | Kuntz Stadium |
| Kansas City Brass | USL League Two | — | — |
| Lafayette SwampCats | Eastern Indoor Soccer League | — | Cajundome |
| Minnesota Twin Stars | National Premier Soccer League | — | Minnetonka High School |
| Sacramento Geckos | USL Second Division | Sacramento | — |
| San Gabriel Valley Highlanders | USL League Two | Glendale | — |
| Savannah Rug Ratz | Eastern Indoor Soccer League | — | Savannah Civic Center |
| Tallahassee Scorpions | Eastern Indoor Soccer League | — | Donald L. Tucker Civic Center |
| Tucson Fireballs | — | Arizona | — |
| Twin Cities Phoenix | USL League Two | — | — |
| Vermont Voltage | USL League Two | Saint Albans | — |
| West Coast FC | Women's Premier Soccer League | — | — |