| AFC Lancaster Lions | American Soccer League | — | Ephrata High School |
| Albion SC San Diego | National Premier Soccer League | San Diego | Mission Bay High School |
| Atletico Baja | Major Arena Soccer League | — | — |
| Boca Raton FC | National Premier Soccer League | Boca Raton | Atlantic Community High School |
| Boston Bolts | USL League Two | — | — |
| CT United FC | — | — | Veterans Stadium |
| Duluth FC | National Premier Soccer League | Duluth | Denfeld High School |
| Evergreen FC | USL League Two | Leesburg | — |
| FC Brownsville | — | Brownsville | — |
| FC Cincinnati | Major League Soccer | Cincinnati | TQL Stadium |
| Florida Tropics SC | Major Arena Soccer League | Lakeland | RP Funding Center |
| Hartford City FC | National Premier Soccer League | Hartford | — |
| Kalamazoo FC | National Premier Soccer League | Kalamazoo | — |
| Kingston Stockade Football Club | National Premier Soccer League | Kingston | Dietz Stadium |
| Kraze United | National Premier Soccer League | — | UCF Soccer and Track Complex |
| Little Rock Rangers | — | Little Rock | — |
| Memphis City FC | USL League Two | Memphis | — |
| Miami FC | USL Championship | Miami | Pitbull Stadium |
| Milwaukee Torrent | National Premier Soccer League | Milwaukee | — |
| Minneapolis City SC | National Premier Soccer League | Minneapolis | — |
| Minnesota United FC | Major League Soccer | Minneapolis | Allianz Field |
| Moreno Valley FC | United Premier Soccer League | — | — |
| New York Cosmos B | National Premier Soccer League | — | Mitchel Athletic Complex |
| Orlando Pride | National Women's Soccer League | Orlando | Inter&Co Stadium |
| Puerto Rico FC | North American Soccer League | Bayamón | Juan Ramón Loubriel Stadium |
| Rayo OKC | North American Soccer League | Oklahoma City | Yukon High School |
| Reno 1868 FC | USL Championship | Reno | Greater Nevada Field |
| Rio Grande Valley FC Toros | USL Championship | Edinburg | Robert and Janet Vackar Stadium |
| Rochester Lancers | — | Rochester | — |
| SoCal SC | — | San Bernardino | — |
| SoCal Surf | USL League Two | Carlsbad | Army and Navy Academy |
| Temple United FC | Texas Premier Soccer League | — | — |
| Tormenta FC | USL League One | Statesboro | Bo Pitts Field |
| Virginia Beach City FC | Women's Premier Soccer League | — | Virginia Beach Sportsplex |