| AC Connecticut | USL League Two | Newtown | — |
| AFC Cleveland | National Premier Soccer League | Independence | Independence High School |
| Arizona Storm | Major Arena Soccer League | Arizona | — |
| Austin Aztex | USL Championship, USL League Two | Austin | House Park |
| Baltimore Bohemians | USL League Two | — | Cedar Lane |
| Bay Area Breeze | USL W-League | Hayward | Pioneer Stadium |
| Boston Victory S.C. | USL League Two | — | Veterans Memorial Stadium |
| Chicago Inferno | USL League Two | — | — |
| Chicago Soul FC | Major Indoor Soccer League | — | Now Arena |
| Chula Vista FC | SoCal Premier League | Chula Vista | Eastlake High School |
| D.C. United U-23 | USL League Two | — | Maryland SoccerPlex |
| Dayton Dutch Lions WFC | Women's Premier Soccer League | — | Dayton Outpatient Center Stadium |
| FC Leones | Puerto Rico Soccer League | — | Estadio Francisco Montaner |
| FC SKBC | — | Pago Pago | Pago Park Soccer Stadium |
| Houston Dutch Lions | USL League Two | The Woodlands | Woodforest Bank Stadium |
| Isabela Soccer Club | Liga Nacional de Fútbol de Puerto Rico | — | — |
| Kansas Magic | Major Arena Soccer League | — | — |
| La Máquina FC | United Premier Soccer League | Santa Ana | — |
| Los Angeles Strikers | USL W-League | — | — |
| MatSu United FC | United Premier Soccer League | — | Palmer High School |
| Mississippi Storm | National Premier Soccer League | — | — |
| Myrtle Beach FC | National Premier Soccer League | Myrtle Beach | Doug Shaw Memorial Stadium |
| New York Fury | Women's Premier Soccer League Elite | — | James M. Shuart Stadium |
| Norfolk SharX | — | — | Norfolk Scope |
| NTX Rayados | — | Dallas | — |
| OC Blues Strikers FC | USL League Two | — | — |
| Philadelphia Union Reserves | MLS Reserve League | — | — |
| SC United Bantams | USL League Two | Columbia | — |
| Seacoast United Mariners | National Premier Soccer League | — | Fitzpatrick Stadium |
| Stade Brestois New York | — | Queensbridge | — |
| Turlock Express | Major Arena Soccer League 2, Major Arena Soccer League | Turlock | — |
| VSI Tampa Bay FC | USL Championship | Plant City | Plant City Stadium |
| VSI Tampa Bay FC | USL League Two | — | Plant City Stadium |
| Wichita Wings | Major Indoor Soccer League | — | Hartman Arena |