| Arizona Rush | Women's Premier Soccer League | Arizona | — |
| Atlanta Silverbacks Reserves | National Premier Soccer League | Atlanta | Atlanta Silverbacks Park |
| Atlantic City Diablos | National Premier Soccer League | — | St. Augustine College Preparatory School |
| Austin Aztex U23 | USL League Two | — | Round Rock High School |
| Black Roses | ASFA Soccer League | Pago Pago | Veterans Field |
| Cary Lady Clarets | USL W-League | — | WakeMed Soccer Park |
| Charm City FC | National Premier Soccer League | — | Arundel High School |
| Chicago Fire NPSL | National Premier Soccer League | — | SeatGeek Stadium |
| Colorado Crimson | National Premier Soccer League | — | — |
| IFC Wild Bills | M*League Division 1 | — | Oleai Sports Complex |
| Illinois Piasa | Major Arena Soccer League | — | — |
| Indianapolis Braves | National Premier Soccer League | — | — |
| Indios USA | — | — | Canutillo Stadium |
| Kalamazoo Outrage | USL League Two | — | Loy Norrix High School |
| Los Angeles Rampage | Women's Premier Soccer League | — | John Elway Stadium |
| Los Angeles Sol | Women's Professional Soccer | Carson | Dignity Health Sports Park |
| Monterey Blues | Women's Premier Soccer League | — | Monterey Peninsula College |
| Morris County Colonials | National Premier Soccer League | — | — |
| NJ-LUSO Rangers FC | USL League Two | — | Morris Catholic High School |
| Northern Colorado Cutthroats | Premier Arena Soccer League | — | — |
| Oklahoma Alliance | Women's Premier Soccer League | — | — |
| Oklahoma City FC | Women's Premier Soccer League | — | — |
| Orlando Sharks | — | Orlando | Amway Arena |
| Pennsylvania Stoners | National Premier Soccer League | Allentown | Whitehall High School |
| Puerto Rico Capitals | Women's Premier Soccer League | — | Parque Yldefonso Solá Morales |
| Puerto Rico United | USL Championship, Puerto Rico Soccer League | Aguada | Aguada Stadium |
| Real Maryland F.C. | USL League Two | Rockville | Richard Montgomery High School |
| Real San Jose | National Premier Soccer League | San Jose | PAL Stadium |
| Rocket City United | National Premier Soccer League | — | — |
| Santa Cruz County Breakers | National Premier Soccer League | Santa Cruz | — |
| SD United | Women's Premier Soccer League | — | Torero Stadium |
| Southern Colorado Stars | Premier Arena Soccer League | — | Colorado Sports Center |
| St. Louis Scott Gallagher Soccer Club | — | — | World Wide Technology Soccer Park |
| Tulsa Spirit | Women's Premier Soccer League | — | — |
| Vitesse Dallas | Premier Arena Soccer League | — | — |