| Atlético de San Juan FC | Puerto Rico Soccer League | San Juan | Hiram Bithorn Stadium |
| Austin Aztex FC | USSF Division 2 Professional League | Austin | House Park |
| Boston Breakers | National Women's Soccer League | Boston | Harvard Stadium, Dilboy Stadium |
| Brave SC | USL League Two | The Villages | — |
| Buffalo City FC | National Premier Soccer League | — | Nichols School |
| Cincinnati Kings Indoor Team | Major Arena Soccer League | — | — |
| Club Deportivo Gallitos | Liga Nacional de Fútbol de Puerto Rico | — | — |
| Colorado Lightning | Major Arena Soccer League | — | Blue Arena |
| Denver Dynamite | Premier Arena Soccer League | — | — |
| Gigantes de Carolina FC | Puerto Rico Soccer League | Carolina | Roberto Clemente Stadium |
| Lancaster Inferno | National Premier Soccer League | — | Hempfield High School |
| Los Angeles Legends | USL W-League | — | — |
| Maine Sting | National Premier Soccer League | — | — |
| MP United F.C. | M*League Division 1 | Northern Mariana Islands | — |
| Philadelphia Union | Major League Soccer | Chester | Subaru Park |
| Pocono Snow | National Premier Soccer League | — | — |
| Portland Timbers U23s | USL League Two | — | Providence Park |
| Richmond Kickers Academy | U.S. Soccer Development Academy | — | — |
| Rio Grande Valley Bravos FC | USL League Two | — | — |
| Rochester Thunder | USL League Two | — | — |
| San Diego United | National Premier Soccer League | — | Torero Stadium |
| San Juan United | Liga Nacional de Fútbol de Puerto Rico | — | — |
| Spartans FC Puerto Rico | Liga Nacional de Fútbol de Puerto Rico | — | — |
| St. Louis Illusion | Major Arena Soccer League | — | — |
| Tampa Bay Rowdies | USL Championship | St. Petersburg | George M. Steinbrenner Field, Al Lang Stadium |
| Tan Holdings FC | M*League Division 1 | — | Ada Gym |
| Texas Outlaws | Major Arena Soccer League | — | — |
| Waza Flo | Major Arena Soccer League | — | Taylor Sportsplex |
| West Texas FC | National Premier Soccer League | Midland | Astound Broadband Stadium |
| Western New York Flash | National Women's Soccer League | Rochester | Marina Auto Stadium |
| Yabucoa Borikén | Liga Nacional de Fútbol de Puerto Rico | — | — |