| Atlanta Blackhawks | USL League Two | — | Alpharetta High School |
| Bay Area Ambassadors | National Premier Soccer League | — | Chabot College |
| Boston Tea Men | — | North Andover | — |
| Cal FC | United States Adult Soccer Association | — | Agoura High School |
| CASL Elite | Capital Area Soccer League | — | WakeMed Soccer Park |
| Eau Claire Aris FC | National Premier Soccer League | — | — |
| Erie Admirals S.C. | National Premier Soccer League | Erie | — |
| F.C. New York | USL Championship, National Premier Soccer League | Queens | Belson Stadium |
| FC Buffalo | USL League Two | Buffalo | Buffalo State University |
| FC Sonic Lehigh Valley | National Premier Soccer League | Whitehall | J. Birney Crum Stadium |
| Fort Lauderdale Schulz Academy | USL League Two | — | — |
| GPS Portland Phoenix | USL League Two | Portland | Memorial Stadium |
| Hollywood United Hitmen | National Premier Soccer League | — | Crenshaw High School |
| Kitsap Pumas | USL League Two | Bremerton | — |
| Louisville Lightning | Major Arena Soccer League | — | — |
| Mass United FC | National Premier Soccer League | — | — |
| Minnesota Kings | National Premier Soccer League | — | — |
| New England Futbol Club | — | Greater Boston | — |
| New Hampshire Mountaineers | National Premier Soccer League | — | — |
| New Jersey Blaze | National Premier Soccer League | — | Rowland Park |
| New York Hakoah | North Jersey Soccer League | New York City | — |
| NorCal Lamorinda United SC | National Premier Soccer League | — | Miramonte High School |
| North Jersey Valkyries | USL W-League | Wayne | DePaul Catholic High School |
| Ohio Vortex | Major Arena Soccer League | — | — |
| Pachuca Puerto Rico | Puerto Rico Soccer League | — | Nestor Morales Stadium |
| Philadelphia Independence | Women's Professional Soccer | Philadelphia | Farrell Stadium |
| Real Madrid | Puerto Rico Soccer League | — | Juan Ramón Loubriel Stadium |
| Red Force FC | American Premier Soccer League | Miami | Tropical Park Stadium |
| Sacramento Gold FC | National Premier Soccer League | — | — |
| San Diego Boca F.C. | National Premier Soccer League | — | Balboa Stadium |
| San Diego Sockers | Major Arena Soccer League | San Diego | Pechanga Arena |
| Santa Ana Winds FC | National Premier Soccer League | Santa Ana | Santa Ana Stadium |
| Saturn FC | National Premier Soccer League | — | — |
| Seattle Sounders FC | Major League Soccer | Seattle | Lumen Field |
| TSC Maryland Red Devils | National Premier Soccer League | — | — |
| Vancouver Whitecaps FC | Major League Soccer | Vancouver | BC Place |
| Washington Freedom Futures | USL W-League | — | Maryland SoccerPlex |