| El Paso Locomotive FC | USL Championship | El Paso | March 1, 2018 | New El Paso Ballpark |
| El Paso Patriots | A-League, USL League Two | El Paso | 1989 | Patriot Stadium |
| El Paso/Juarez Gamecocks | United Soccer League | — | 1984 | Estadio Olímpico Benito Juárez |
| Elizabeth S.C. | Cosmopolitan Soccer League | Union County | 1924 | — |
| Emigrantes das Ilhas | United States Adult Soccer Association | Brockton | 1987 | — |
| Erie Admirals S.C. | National Premier Soccer League | Erie | 2009 | — |
| Evansville Crush | Premier Arena Soccer League | — | 2010 | Metro Sports Center |
| Evergreen Diplomats | American Soccer League | — | 2014 | — |
| Evergreen FC | USL League Two | Leesburg | 2015 | — |
| Excelsior of Brooklyn | National Association of Base Ball Players | — | 1854 | — |
| F.C. Indiana | Premier Arena Soccer League | Lafayette | 2000 | — |
| F.C. Indiana | Women's Premier Soccer League Elite | — | 2000 | — |
| F.C. New York | USL Championship, National Premier Soccer League | Queens | 2009 | Belson Stadium |
| Fajardo FC | Puerto Rico Soccer League | — | 2010 | — |
| Fall River F.C. | — | Fall River | 1931 | Mark's Stadium |
| Fall River Rovers | — | Fall River | 1884 | — |
| Fall River S.C. | American Soccer League | Fall River | 1957 | — |
| FC Arizona | National Premier Soccer League | Mesa | 2016 | — |
| FC Baltimore Christos | — | Baltimore | 2018 | — |
| FC Brownsville | — | Brownsville | 2015 | — |
| FC Buffalo | USL League Two | Buffalo | 2009 | Buffalo State University |
| FC Cincinnati | Major League Soccer | Cincinnati | August 12, 2015 | TQL Stadium |
| FC Columbus | National Premier Soccer League | Columbus | — | — |
| FC Dallas | Major League Soccer | Frisco | 1995 | Toyota Stadium |
| FC Denver | Premier Arena Soccer League | Denver | 2005 | — |
| FC Force | National Premier Soccer League | — | 2014 | Central Union High School |
| FC JAX Destroyers | USL League Two | — | 2010 | D. B. Milne Field |
| FC Kansas City | National Women's Soccer League | Kansas City | 2012 | Shawnee Mission District Stadium, Sporting Park, Swope Soccer Village, Durwood Soccer Stadium |
| FC Leones | Puerto Rico Soccer League | — | 2011 | Estadio Francisco Montaner |
| FC Miami City | USL League Two | Miami | 2014 | Tropical Park Stadium |
| FC Monmouth | National Premier Soccer League | Monmouth County | 2017 | — |
| FC Motown | National Premier Soccer League | Madison | 2012 | — |
| FC Naples | USL League One | — | January 2024 | — |
| FC New Orleans | National Premier Soccer League | — | 2012 | — |
| FC Santa Clarita | National Premier Soccer League | — | 2006 | — |
| FC SKBC | — | Pago Pago | 2011 | Pago Park Soccer Stadium |
| FC Sonic Lehigh Valley | National Premier Soccer League | Whitehall | 2009 | J. Birney Crum Stadium |
| FC Tacoma 253 | National Premier Soccer League | — | 2014 | — |
| FC Tucson | USL League One | Arizona | 2010 | Veterans Memorial Stadium |
| FC Tulsa | USL Championship | Tulsa | December 2013 | Oneok Field |
| FC Wichita | National Premier Soccer League | Wichita | 2013 | — |
| Fire 98 SC | Women's Premier Soccer League | — | 2018 | — |
| FK Pacific | Pacific Coast Soccer League | — | 2003 | Starfire Sports |
| Flint City Bucks | USL League Two | Flint | 1995 | Ultimate Soccer Arenas |
| Florida Tropics SC | Major Arena Soccer League | Lakeland | 2015 | RP Funding Center |
| Fort Lauderdale Schulz Academy | USL League Two | — | 2009 | — |
| Fort Lauderdale Strikers | North American Soccer League | Fort Lauderdale | 1977 | Lockhart Stadium |
| Fort Lauderdale Strikers | North American Soccer League | Fort Lauderdale | 2006 | Lockhart Stadium |